Coding

Part:BBa_K1111006

Designed by: Luisa Spisak, Marcelle Isaline Arrigo   Group: iGEM13_EPF_Lausanne   (2013-09-19)

Matrix metalloprotease 2 (MMP2)

This enzyme is supposed to degrade gelatin. Its coding sequence was extracted from a plasmid.med.Harvard.edu plasmid by PCR and a His tag was added at the beginning of the sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 429
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None